Amphotericin B induces interdigitation of apolipoprotein stabilized nanodisk bilayers
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چکیده
منابع مشابه
Amphotericin B induces interdigitation of apolipoprotein stabilized nanodisk bilayers.
Amphotericin B nanodisks (AMB-ND) are ternary complexes of AMB, phospholipid and apolipoprotein organized as discrete nanometer scale disk-shaped bilayers. In gel filtration chromatography experiments, empty ND lacking AMB elute as a single population of particles with a molecular weight in the range of 200 kDa. AMB-ND formulated at a 4:1 phospholipid:AMB weight ratio separated into two peaks. ...
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Objective Amphotericin B (AMB), a potent antifungal agent, has been employed as topical and systemic therapy for sinonasal fungal infections. A novel formulation of nanodisc (ND) containing super aggregated AMB (ND-AMB) for the treatment of fungal infections has been recently developed to provide greater protection from AMB toxicity than current, clinically approved lipid-based formulations. Th...
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The antimycotic activity of amphotericin B (AmB) depends on its ability to make complexes sterols to form ion channels that cause membrane leakage. To study this phenomenon, surface pressure (pi) as a function of surface area (A) and pi-A hysteresis were measured in monolayers of AmB-cholesterol mixtures on the water-air interface. The most stable monolayers were produced from molecules of AmB ...
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The antimycotic activity of amphotericin B depends on its ability to make complexes with cell membrane sterols. Surface pressure (π) as a function of surface area (A) and π-A hysteresis were measured for monolayers of amphotericin B/cholesterol mixtures on the water/air interface. Specific area per molecule of amphotericin B and free energy of mixing were calculated as a function of concentrati...
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OBJECTIVES NanoDisk-amphotericin B (ND-AMB) is a protein-phospholipid bioparticle containing a "super aggregate" form of antifungal AMB. While lipid-based formulations of AMB, including liposomal AMB (L-AMB), are safer than the deoxycholate (DOC) solubilized form (DOC-AMB), the potency of lipid-based formulations is attenuated. We have developed an AMB-based therapy that is both well tolerated ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2008
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2007.10.005